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KMID : 0603820130190030261
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Journal of Experimental & Biomedical Science
2013 Volume.19 No. 3 p.261 ~ p.265
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Endothelial Cells Isolated from the Bovine Corpus Luteum Synthesize Prostaglandin F2α Receptor
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Gwon Sun-Yeong
Rhee Ki-Jong
Lee Seung-Hyung
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Abstract
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The corpus luteum is a transient endocrine gland essential for regulation of the ovarian cycle as well as for establishing
and maintaining pregnancy. Prostaglandin F2α (PGF) initiates functional and structural regression of the corpus luteum
and therefore is an important regulator of the estrous cycle. It is a matter of debate whether the endothelial cells of
the bovine corpus luteum express PGFR, the cognate receptor for PGF. Therefore, the aim of this study was to assess
the expression of PGFR in bovine endothelial cells. Endothelial cells were isolated from the bovine corpus luteum of
the mid?luteal stage using magnetic beads and cultured in vitro. We demonstrate that this isolation procedure generates
a pure culture of endothelial cells as confirmed by synthesis of Factor VIII and lack of expression of 3β?hydroxysteroid
dehydrogenase. By RT?PCR, Western blot and immunofluorescence analyses, we further show that the cultured endothelial cells produced PGFR. This model system can be utilized to provide an experimental system to investigate the role of PGF on endothelial cells during the reproductive cycle.
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KEYWORD
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Prostaglandin F2α (PGF), PGF receptor (PGFR), Endothelial cell, Corpus luteum
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